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Full Paper

Synthesis, Reductive Cleavage, and Cellular Interaction Studies of Biodegradable, Polyglycerol Nanogels

Abstract

In this paper we describe disulfide containing, polyglycerol nanogels as a new class of biodegradable materials. These nanoparticles are prepared in inverse miniemulsion via an acid catalyzed ring‐opening polyaddition of disulfide containing polyols and polyepoxides. Varying conditions allow us to tune particle size and disulfide content within the polymer network; particles can be prepared with narrow polydispersities and diameters in the range from 25 to 350 nm. Particle degradation under reductive intracellular conditions is studied by various analytical techniques. Gel permeation chromatography indicates that final degradation products have relatively low molecular weights (≤ 5 kDa). In addition, studies in cell culture show these nanoscale materials to be highly biocompatible. Dye‐labelled nanogels are shown by optical microscopy techniques to readily internalize into cells by endocytotic mechanisms. This study highlights the great potential of these particles to function as sophisticated nanotransporters that deliver cargo to a certain tissue or cell target and then biodegrade into smaller fragments which would be cleared from the body by the kidney. (with ≈ 30 kDa molecular weight cut off)

Citing Literature

Number of times cited according to CrossRef: 65

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Born in Dallas, Texas in 1934, Janet Banzet couldn't have started off her career any better, appearing alongside Audrey Hepburn in the classic film Breakfast at Tiffanys (1961). A career in mainstream films just wasn't in the cards for Janet, however, as the allure of a quick and easy buck called her name and she plunged down the rabbit hole of the early skin flick movement. She appeared nude in no fewer than a dozen films including The Lusting Hours (1967), Mini-Skirt Love (1967), Smoke and Flesh (1968), A Thousand Pleasures (1968), The Ultimate Degenerate (1969), all of which we have screen caps and some videos featuring Janet. Our favorite role of hers was in 1970's Italian Stallion, aka The Party at Kitty and Stud's, made famous for its appearance from Sylvester Stallone in an X-rated film. Check out Janet flashing full frontal flappers and fur under her fur coat out in the park! Why not pound a side of beef in honor of Janet Banzet in Italian Stallion!

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The Ultimate Degenerate (1969) Nude, breasts 00:51:00 Janet gets topless and airs out her rutabagas while fellating some corn on the cob. (1 min 8 secs)

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Italian Stallion (1970) Nude, breasts, bush 00:23:00 As Sly Stallone drives away, Janet opens her coat and shows an unbelievably lush frontal flash. (14 secs)

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The Lusting Hours (1967) Nude, breasts 00:24:00 Janet (in the black panties) relaxes on the bed, tits out, while her lez love Sheila Britt loses her laundry for some sexy clam-jousting. (2 mins 10 secs)

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